Figure 7. Constitutively activate CaMKII mimics effects of morphine on GIRK signaling.
20 DIV hippocampal neurons were infected with virus expressing constitutively active CaMKII (ΔCaMKII-GFP). A,B) Representative images of dendrites 1-day after infection. Low power images show location of infected and uninfected dendrites immunostained for GIRK2 and PSD95 (A). Gray scale image shows GFP-channel. B) Zoom of dendrites immunostained for GIRK2 and PSD95 from infected and uninfected neurons. Scale bars: 10 and 2 µm. C) Whole-cell patch-clamp recording from representative neuron infected with ΔCaMKII-GFP (11–14 DIV). The response to baclofen and Ba2+ are shown. Arrow indicates EK. D) Bar graphs show mean (± SEM) amplitude for baclofen-induced (left) and Ba2+-sensitive basal K+ currents (right) for uninfected and infected neurons. *Student’s t-test for significance (P < 0.05).