TABLE 5.
Effect of the pho operon on PitB inorganic phosphate uptake activity
| Strain | Relevant genotype | Alkaline phosphatase activitya | Growth on minimal medium with source of phosphate:
|
Phosphate uptaked | |
|---|---|---|---|---|---|
| Pi and G3Pb | Pic | ||||
| AN3066 | pitB+phoB+phoR+ | + | + | − | 0.6 ± 0.1 |
| AN4081 | pitB+ | − | + | + | 4.4 ± 0.3 |
| AN4085 | − | + | + | 0.9 ± 0.2 | |
a
Rapid spray assay on cells grown on Luria-Bertani medium.
b
500 μM Pi and 1 mM G3P.
c
500 μM Pi.
d
Assayed at 20 μM Pi. Values are shown with standard errors of the means. n = 4. Values are nanomoles of Pi minute−1 milligram (dry weight)−1.