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. 2021 Jun 16;144(6):487–498. doi: 10.1161/CIRCULATIONAHA.121.056038

In-Depth Evaluation of a Case of Presumed Myocarditis After the Second Dose of COVID-19 mRNA Vaccine

Alagarraju Muthukumar 1, Madhusudhanan Narasimhan 1, Quan-Zhen Li 2,3, Lenin Mahimainathan 1, Imran Hitto 1, Franklin Fuda 1, Kiran Batra 4, Xuan Jiang 3, Chengsong Zhu 3, John Schoggins 5, James B Cutrell 3, Carol L Croft 3, Amit Khera 3, Mark H Drazner 3, Justin L Grodin 3, Benjamin M Greenberg 6,7, Pradeep PA Mammen 3, Sean J Morrison 8,9, James A de Lemos 3,✉
PMCID: PMC8340727  NIHMSID: NIHMS1717465  PMID: 34133883

Abstract

Supplemental Digital Content is available in the text.

Keywords: COVID-19, COVID-19 vaccine, myocarditis, vaccine

Initial Presentation

A 52-year-old man presented to the emergency department ≈90 min after the onset of substernal chest pain. Three days before presentation, he received his second dose of mRNA-1273 (Moderna) vaccine for coronavirus disease 2019 (COVID-19), and the next day had a severe reaction that he described as being the “worst he had ever felt.” He had subjective high fevers, shaking chills, myalgias, and a headache. These symptoms largely resolved by the third day after vaccination except for a positional headache that was unusual for him. On the morning of hospitalization, he walked 3 to 4 miles and felt fine. Later that day, while in a meeting, he developed persistent midsternal chest discomfort without radiation, prompting him to seek evaluation in a university hospital emergency department. The pain subsided spontaneously after approximately 3 hours. He had no associated dyspnea, palpitations, dizziness, fever, chills, or myalgia.

The patient had a past medical history of hypertension, hypercholesterolemia, obstructive sleep apnea treated with an oral appliance, and minor elevations in liver function tests attributed to possible hepatic steatosis. A recent screening coronary artery calcium scan demonstrated coronary artery calcium at the 81st percentile for age and sex. The patient had no previous history of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. His medications included aspirin 81 mg, simvastatin 40 mg, ezetimibe 10 mg, and lisinopril 10 mg daily, and he took no supplements. He drank alcohol socially and denied use of tobacco and all recreational drugs.

On physical examination, the following vital signs were recorded: oral temperature 36.8°C, pulse 73/min, blood pressure 124/76, and respiratory rate 18/min, and his oxygen saturation was 100% on room air. Pulmonary and cardiac examinations were normal without a pericardial friction rub. The remainder of his physical examination was normal.

In the emergency department, his initial ECG showed sinus rhythm with left axis deviation and incomplete right bundle-branch block without ST or T wave changes (Figure 1A). His initial high-sensitivity cardiac troponin I was 2768 ng/L. Point-of-care echocardiogram showed normal left ventricular function and volumes, and no wall motion abnormalities. Urgent coronary angiography showed mild nonobstructive coronary artery disease with no stenoses or visible thrombus and no evidence of coronary embolism or dissection (Figure 1B and 1C).

Figure 1.

Figure 1.

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ECG and coronary angiogram. A, The ECG on presentation to the emergency department. B, A posterior anterior cranial projection of a dominant right coronary artery and with no severe angiographic stenoses or flow-limiting lesions in the main vessel or its branches. C, A right anterior oblique caudal projection of a bifurcating left coronary artery and no severe angiographic stenoses or flow-limiting lesions in the main vessel or its branches.

His initial laboratory panel revealed normal white blood cells 6.3 × 109/L (76% polymorphonuclear leukocytes, 14% lymphocytes, 9% monocytes, 0.5% eosinophils, and 0.2% basophils), hemoglobin 14.9 g/L, and platelets 207 × 109/L. Chemistries were remarkable for glucose of 172 mg/dL, but creatinine 0.87 mg/dL and alanine aminotransferase 58 U/L were consistent with his baseline. High-sensitivity cardiac troponin I peaked at 6770 ng/L at 7 hours after admission and remained elevated (551 ng/L) even after 4 days. In contrast, high-sensitivity cardiac troponin T and creatine kinase-MB biomarkers showed modest elevation (Table 1). C-reactive protein, erythrocyte sedimentation rate, and D-dimer were elevated in the first sample taken at the time of admission but resolved to near normal levels within 1 to 2 days. Antinuclear antibodies were negative.

Table 1.

Relevant Biochemical Parameters in the Case of Interest

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Additional Clinical Testing

A repeat echocardiogram performed on hospital day 2 revealed a normal ejection fraction without wall motion abnormalities and no valvular or pericardial abnormalities. Contrast-enhanced cardiac magnetic resonance imaging (MRI) with parametric mapping was performed on a 1.5T MRI scanner (Siemens Healthineers) on hospital day 3. Delayed contrast-enhanced phase-sensitive images showed midmyocardial and subepicardial linear and nodular late gadolinium enhancement in the inferoseptal, inferolateral, anterolateral, and apical walls. The left ventricle showed mild dilatation and “low normal” left ventricular ejection fraction at 54%. The right ventricular ejection fraction was normal at 58%. Parametric mapping showed elevated T1 relaxation time and relative inhomogeneity and focal elevation of the T2 relaxation values (Figure 2). In addition, wall motion abnormalities with mild hypokinesis of the lateral and inferior apical walls were noted. These findings were consistent with myocarditis on the basis of the modified Lake Louise criteria.1

Figure 2.

Figure 2.

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Phase-sensitive inversion-recovery cardiac magnetic resonance imaging. Right, Short-axis views demonstrating linear and curvilinear delayed enhancement in the subepicardial inferior basal and mesocardial midventricular region, compatible with nonischemic pattern of delayed enhancement. Middle, A native T1 map showing globally increased T1 values (1054 ms), Local native myocardial T1 (short axis [SA] and 4 chamber [4CH] midwall) (965 ± 35) and specifically higher values in the regions of delayed enhancement. The color map shows relaxation times with normal relaxation time in green and increased relaxation time in red and orange. Left, Native T2 map with heterogenous relative increased T2 values within the same segments (arrows) (maximum T2 value was 65 ms) (local normal T2 values for our institution, 45–64 ms). Color scale shows time in milliseconds.

Hospital Course

The chest discomfort in the patient had fully resolved within 3 hours after onset and did not recur. He reported feeling normal throughout the remainder of his 4-day hospital stay. Endomyocardial biopsy was not performed because of his resolution of symptoms, preserved left ventricular ejection fraction, and the absence of any hemodynamic or arrhythmic complications. The patient was treated with low-dose lisinopril and carvedilol, but no immunosuppressive or anti-inflammatory medications. At the time of discharge, the patient remained asymptomatic, and his high-sensitivity cardiac troponin T levels had fallen to 138 ng/L. His NT-proBNP (N-terminal pro-B-type natriuretic peptide) at discharge was <27 pg/mL.

Postdischarge Course

The patient has had no recurrent symptoms in >3 months since hospital discharge. Given the presumptive diagnosis of myocarditis, exercise has been restricted, and he has remained on β-blocker and angiotensin-converting enzyme inhibitor medications. Repeat high-sensitivity cardiac troponin I was 10 ng/L 4 days after discharge and undetectable (<5 ng/L) 2 weeks after discharge. Serial cardiac MRIs have been performed showing a gradual reduction in left ventricular volumes and reduction in the degree of late gadolinium enhancement abnormalities, with normalization of T1 relaxation time and decrease in the T2 relaxation time (Table 2).

Table 2.

Left Ventricular Volume and Late Gadolinium Enhancement Abnormalities in the Case of Interest

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Exploratory Studies to Investigate Potential Pathological Mechanisms

Myopericarditis has been reported to the US national passive Vaccine Safety Surveillance System (VAERS) as a rare adverse event after vaccinations, with most reports associated with smallpox vaccination.2 However, at the time of the patient’s presentation, there were no reported cases of myocarditis caused by COVID-19 vaccination.

To explore potential mechanisms of myocardial injury in temporal association with vaccination in the present case, written informed consent was obtained for additional in-depth analysis of viral, cytokine, and autoimmune panels and subsequent research publication of the case. Samples from the patient of interest were compared with excess, remnant blood specimens that were available in the laboratory after routine clinical testing. Samples from the case of interest (CI) were collected on days 1 to 4 after symptom onset (CIS1–S4) and were compared with 4 groups: naive unvaccinated (NUV; n=8), unvaccinated patients hospitalized with COVID-19 (n=10), naive vaccinated (NV; n=10), and age-matched controls receiving Moderna vaccine (NM, n=2). NV and NM groups were tested ≈?2 weeks after receiving their second vaccine dose. The studies were performed as part of a biorepository protocol approved by the University of Texas Southwestern Institutional Review Board, and waiver of Institutional Review Board consent was obtained to use the remnant blood specimens. Detailed methods are provided in the Methods in the Data Supplement.

Results of Exploratory Studies

Antibody response to viral antigens and SARS-CoV-2 nucleocapsid and spike proteins serum immunoglobulin (Ig) G antibodies against 18 different viral antigens and SARS-CoV-2 serology testing were measured using a custom developed proteome array and US Food and Drug Administration–approved standard assays, respectively, using the methods described in the Methods in the Data Supplement. These studies confirmed the absence of previous COVID-19 infection (negative reactivity for SARS-CoV-2 nucleocapsid IgG) (Figures 3 and 4). As expected, a clear immune response to the vaccine (SARS-CoV-2 spike as a component) was observed in the case of interest 5 and 6 days after the second dose of Moderna vaccine, which corresponds with the third and fourth day after symptom onset in the case of interest (CIS3 and CIS4) (Figure 3). Comparison of the strength of vaccine-induced immune responses in the case of interest at the measured sampling period CIS2 and CIS4 with either NV or NM did not reveal abnormally elevated SARS-CoV-2 spike IgG or SARS-CoV-2 spike IgM levels (Figure 4). Low IgG serology reactivity was noted in the case patient samples (CIS1–S4) for the other viral antigens, including cytomegalovirus, Epstein-Barr virus, influenza A, and respiratory syncytial virus compared with vaccinated control samples (Figure 3). It is interesting that, although anti-spike antibody levels were higher than the manufacturer-recommended positive threshold, the SARS spike protein antibody levels were either lower or just comparable in the case compared with NV (Figure 4). This may be partly explained by a difference in the timing of blood sampling after immunization among the vaccinated controls (2 weeks) versus case patient (5–6 days) for assessing antibody response. Concurrent clinical evaluation for known infectious causes of acute myocarditis, including multiple SARS-CoV-2 nasopharyngeal polymerase chain reaction tests and Food and Drug Administration–approved multiplex respiratory viral polymerase chain reaction panels and serologies, were all negative with 2 exceptions. An IgG antibody for Mycoplasma pneumoniae was positive but IgM antibody was negative, consistent with previous exposure and not acute infection. In addition, an IgG titer of 1:320 was reported for coxsackie B virus 4, but IgM antibody titers were negative. However, convalescent serum antibody testing 3 weeks later revealed a titer of 1:160, consistent with remote and not acute or recent infection.

Figure 3.

Figure 3.

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Antibody profile to viral antigens in the case of interest as compared with naive vaccinated, naive unvaccinated, and COVID-19 unvaccinated patients. The heatmap shows immunoglobulin G reactivity expressed in terms of row z-score for a respective antigen across different patient samples. Each antigen is organized into rows color-coded by virus, for serum specimens organized into columns classified as naive unvaccinated (NUV, 8 samples), (COVUV, 10 samples), naive vaccinated (NV, 10 samples), naive Moderna vaccinated controls (NM, 2 samples), and case of interest samples (CIS, collections at day 1, day 2, day 3, and day 4 after symptom onset: S1, S2, S3, and S4 in the respective order). Reactivity is represented by color (light blue=low, black=mid, ellow=high). The heatmap has normalized row z-score values, a typical scaling method that helps better visualization of analytes with varying trends in the expression/reactivity between samples. Although a normalized row z-score can better represent the nonrandomness of directionality within a dataset, a negative z-score does not indicate a complete absence of expression/reactivity. A negative z-score means comparatively a lower raw scores/absolute expression. CMV indicates cytomegalovirus; COVID-19, coronavirus disease 2019; EBV, Epstein-Barr virus; and RSV, respiratory syncytial virus.

Figure 4.

Figure 4.

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SARS-CoV-2–related antibody status in the case of interest as compared with naive vaccinated, naive unvaccinated, and COVID-19–unvaccinated patients. Comparison of SARS-CoV2–related antibody response in the case of interest with naive vaccinated, naive unvaccinated, and COVID unvaccinated patients. A, Evaluation of spike-specific IgG antibody response. B, Comparison of spike-specific IgM levels. C, Comparison of nucleocapsid-specific antibody response. For A through C, all the patient samples in the NV group were immunized with Pfizer vaccine. AU indicates arbitrary units; CIS2, case of interest sampled at day 2 after symptom onset; CIS4, case of interest sampled at day 4 after symptom onset; COVID-19, coronavirus disease 2019; COVUV, COVID-19 unvaccinated; Ig, immunoglobulin; NM, age- and vaccine (Moderna)–matched naive (positive controls for case of interest); NUV, naive unvaccinated; NV, naive vaccinated; SARS-CoV2, severe acute respiratory syndrome coronavirus 2; and SP, spike. Dashed brown line indicates the manufacturer-recommended positive threshold of the respective antibody assays used.

Genetic Testing

Given that inherited cardiomyopathy may present clinically as myocarditis,3 a panel test for variants in 121 genes potentially linked to cardiomyopathy was performed (Invitae, San Francisco, CA). No pathogenic variants and 1 intronic variant of unknown significance (heterozygous, ACTN2, c2367+5G>A) were identified, suggesting that the known gene variants are not the cause of myocarditis in the case patient.

Screening of Cytokine Response

Although the vaccine-induced immune response is chiefly linked to protective immunity, an exaggerated and unwarranted immune reaction could potentially heighten inflammation and augment the risk of immunopathology. We measured a panel of 48 cytokines and chemokines in the case patient using fluorescent bead-based Bio-Plex Pro Human Cytokine Screening Panel, per the manufacturer’s instructions (Bio-Rad, CA), as described in the Methods in the Data Supplement. Cytokine levels in the case patient were NV or NM (Figure 5). The trend of cytokine changes in the case of interest along with the control groups is shown in Table 3. To aid efficient interpretation of this data, we considered as abnormal only the analytes with ≥2.0-fold increase (bold) or a ≥2.0 decrease (bold and italics) in CIS1–S4 versus both NM and NUV groups. The NUV comparison provides a reference interval to interpret the case patient’s cytokine results. Given the inclusion of 2 comparators NM and NUV, if the 2-fold change is in 1 direction versus 1 comparator and in the opposite direction for another comparator, then those cytokine changes are indicated in italics. This analysis revealed in the case patient elevated levels of 4 cytokines (IL-1ra, IL-5, IL-16, and MIG), diminished levels of 1 cytokine LIF (leukemia inhibitory factor), and 3 other cytokines (IL-10, MIF, and VEGF) with bidirectional pattern (increase or decrease) relative to the comparators, NM or NUV (Table 3). Although statistical inference is not possible because of the single case patient, and the clinical relevance of the magnitude of difference seen is not clear, some of the following changes are of potential interest. The level of IL-1ra (IL-1 receptor antagonist) in the first sample from the case patient after symptom onset (CIS1; 1174 pg/mL) was comparable with levels in patients with active COVID-19 infection (unvaccinated patients hospitalized with COVID-19; 1183 pg/mL). Generation of IL-1ra could be a compensatory counterattacking mechanism to limit excessive inflammation. In support of this notion, it has been documented that treatment with IL-1ra rescues myocarditis-associated end-stage heart failure.4 Around the time of symptom onset, the case patient also displayed elevated levels of other cytokines, IL-5, IL-16, and MIG (CXCL9), which play inflammatory roles in either myocarditis or related cardiac complications in humans or in experimental animal models.5–8 In contrast, relative to NM or NUV, the first sample of the case patient (CIS1) showed a decrease in the levels of cytokine LIF, which provides cellular stability and ensures survival of cardiomyocytes during stress.9 The other 3 cytokines, VEGF, IL-10, and MIF, did not reveal a unidirectional regulatory pattern with comparators (NM and NUV); however, each spiked above the NUV reference group and has been individually implicated in immune vasculitis.10–12 Additional clinical laboratory assessment of IL-1β, IL-2, and IL-6 cytokines revealed normal levels of these cytokines (data not shown).

Figure 5.

Figure 5.

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Cytokine profile in the case of interest as compared with naive vaccinated, naive unvaccinated, and COVID-19–unvaccinated patients. The heatmap shows reactivity expressed in terms of row z-score for a respective antigen across different patient samples. Each row in the graphics represent a cytokine for serum specimens organized into columns classified as naive unvaccinated (NUV, 8 samples), COVID-19 unvaccinated (COVUV, 10 samples), naive vaccinated (NV, 10 samples), naive Moderna vaccinated controls (NM, 2 samples), and case of interest samples (CIS, 4 different collection days at day 1, day 2, day 3, and day 4 after symptom onset: S1, S2, S3, and S4 in the respective order). The reactivity intensity ranges from turquoise (low) to black (moderate) or yellow (high). For the groups NM and CIS, each patient sample was run in duplicates that were averaged and represented. Some of the samples that displayed values below the least detection range were arbitrarily assigned a lowest value. COVID-19 indicates coronavirus disease 2019.

Table 3.

Cytokine Profile in Naive Unvaccinated, COVID-19 Unvaccinated, Naive Vaccinated, and the Case of Interest

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It should be emphasized that these cytokine analyses are exploratory and limited by the absence of baseline measurements in the case patient before vaccination. Although this empirical evidence cannot identify a specific cytokine candidate or signature, this approach represents a first step of searching for such a cytokine signature in COVID-19 vaccine–associated myocarditis and may provide important insights for subsequent studies in larger numbers of patients.

Autoantibodies

Immunizations with adverse effects typically induce disproportionate autoantibody generation.13,14 Thus, we next investigated whether the COVID-19 mRNA vaccine and the associated nonviral acute myocarditis seen in the patient of interest may be a consequence of an autoimmune response, using a proteome array printed with HuProtTM version 3.1 arrays (CDI Laboratories, Mayaguez, PR) comprised of ≈19,500 unique full-length human proteins (Methods in the Data Supplement).

Analyses for potentially informative autoantibodies were clustered into 3 separate subpanels representing common, COVID-specific, and CIS-specific groups for both IgM and IgG classes of circulating autoantibodies (Figure 6A and 6B). In the common subpanel, the case patient was characterized by higher levels of 2 IgM autoantibodies (CRK and UNC45B) (Figure 6A) and 6 IgG autoantibodies (IL-10, KCNK5, PARP1, VCL, AKAP5, and IFNγ) compared with the patient with active COVID-19 and NUV controls (Figure 6B), suggesting potential specific associations with myocarditis. Autoantibodies against IL-10 and IFNγ have been detected in patients with life-threatening COVID-19, and previous reports indicate a cardioprotective effect for these cytokines in humans and rodents.15–17 IgM autoantibodies against several common antigens, including TNNC1 (troponin C1) and IL-1RN, were elevated in both the case patient and the patient with COVID-19, which is expected given the presence of cardiac injury and inflammation present in both disease scenarios.

Figure 6.

Figure 6.

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Antibody profiles to self-antigens in the case patient relative to unvaccinated naive and COVID-19 patient samples. The heatmap shows the Phenolyzer-prioritized candidate proteins involved in cardiac disease expressed in terms of mean of the individual signal intensities from the duplicate samples that were corrected for background intensity followed by variance stabilizing normalization (VSN). Each row in the graphics represent the analytes for serum specimens organized into columns classified as naive unvaccinated (NUV, 2 samples), COVID-19 unvaccinated (COVUV, 1 sample) and case of interest samples (CIS; day 1 sample after symptom onset). The reactivity intensity ranges from blue (low) to white (moderate) or red (high). Horizontal black lines segregate the subpanel cluster where autoantibodies are altered either commonly in both COVUV and CIS or only in COVUV or CIS relative to NUV. A, IgM-specific autoantibody changes. B, IgG-specific autoantibody changes. Abs indicates antibodies; COVID-19, coronavirus disease 2019; and Ig, immunoglobulin.

In the CIS-specific cluster, the case patient (CIS) had a pronounced excess of 3 IgM (CCDC97, CDK6, and EPHX2) and 21 IgG (AK1, CIRBP, CKM, CTGF, CXCL16, DGKZ, DNAI1, DNAI2, GDI1, HIP1R, HSPA9, IFT122, JUNB, KIF6, PQBP1, SF3A2, SH3GL2, STAMBP, THBD, TSEN34, and XXYLT1) specific autoantibodies compared with NUV or unvaccinated patients hospitalized with COVID-19 (Figure 6A and 6B). Out of this list, CXCL16 protein has been shown to increase in acute versus chronic myocarditis and suggested to be a novel biomarker for inflammatory cardiomyopathy.18 Likewise, elevated levels of connective tissue growth factor (CTGF/CCN2) have been elevated in fibrotic and tissue injury in heart failure.19 In addition, CIRBP/CIRP (cold-inducible RNA binding protein), a known cardiac electrophysiological regulator, has been shown to govern ventricular and atrial repolarization on cellular stress.20 The absence of antibody levels exceeding the prespecified criteria that cross-react with cardiac myosin and first and second extracellular loops of the β-adrenergic receptor is notable given previous reports of these autoantibodies in viral myocarditis.21,22 Whether the specific autoantibodies identified in the case patient play a role in disease progression or resolution needs to be determined by assessing their dynamics over longer-term follow-up in studies with larger sample sizes.

This exploratory autoantibody analysis encompasses changes in autoantibodies against both intracellular and extracellular proteins/targets (Figure 6). However, it should be noted that autoantibodies against extracellular proteins/targets are more plausibly linked to disease pathology, in part because of their easy accessibility for binding.23 Also, autoantibodies to extracellular proteins are frequently reported to mimic genetic diseases for the same target protein or pathway with corresponding gain or loss of function.24 When autoantibodies against intracellular proteins are linked to a pathogenesis, it is often indirect, because the clonal expansion and the related process of recognition of antigens through the B-cell receptor occur in extracellular space with the support of extracellular proteins.25 Nevertheless, the autoimmune reactivity seen in the patient with respect to these self-antigens need not necessarily be pathogenic and could also be a part of the normal healing process of the inflamed myocardium. Future studies that specifically characterize the function and origin of these autoantibodies will be essential to understand their potential role in vaccine-associated myocarditis. It is ideal for such future studies to include a baseline sample from the same patient and also age-, sex-, and vaccine type–matched controls.

Immune Cell Subsets

Last, we assessed alterations in immune cell subsets in the patient of interest by enumerating T, B, and natural killer (NK) lymphocytes and investigating immunophenotypic aberrancy. This was performed using Becton Dickinson’s Multitest 6-color lymphocyte subsetting reagent with Becton Dickinson Trucount and additional immunophenotyping (CD3, CD4, CD5, CD7, CD8, CD10, CD11b, CD13, CD14, CD16, CD19, CD20, CD22, CD25, CD31, CD34, CD38, CD45, CD45RA, CD62L, CD64, CD123, CD127, CD197, HLA-DR, TCRαβ, TCRγδ) by flow cytometry on a Becton Dickinson FACSCanto. Table 4 demonstrates that a naive vaccinated subject had a frequency of CD3negCD16posCD56pos NK cells within the normal reference range. In contrast, the case patient had a nearly 2-fold increase in the frequency of CD3negCD16posCD56pos NK cells from the upper limit of the reference interval. This may indicate activation of a distinct subset of NK cells (CD3negCD16posCD56pos) that have been documented to be the most abundant (10% peripheral blood lymphocytes) and efficient cytotoxic effectors that kill their target cells by secreting cytoplasmic proteins.26 Although the changes in the absolute NK cell number are intriguing at present, the surge may have either contributed to the pathology or the disease resolution process. Low absolute CD3negCD16posCD56pos NK cell counts have been shown to correlate with orbital myositis, and the levels of these cells normalized with improvement in the disease activity.24 In addition, the patient showed a marginal increase in percentage and absolute count of NK lymphocytes by about 17% relative to the NM sample. This finding of high NK cell fraction in the case patient with resolved myocarditis contrasts with the recent multicenter IPAC study (Investigation of Pregnancy-Associated Cardiomyopathy), which showed reduced levels of NK cells in peripartum cardiomyopathy, which normalized over time postpartum.27 Although the significance of this finding in the current patient with nonviral myocarditis is unclear, NK cells are known to play a cardioprotective role in viral myocarditis and autoimmune myocarditis by limiting viral replication and through modulation and inhibition of cardiodestructive activity by eosinophils, respectively.28 No other disease-driving immunophenotypic aberrancies were noted in the patient.

Table 4.

Enumeration of T, B, and NK Lymphocyte Subsets in the Case of Interest Relative to Age- and Vaccine-Matched Naive Subject

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Discussion

This case describes a 52-year-old previously healthy man who presented with an acute myocarditis-like illness 3 days after the administration of the second dose of Moderna’s COVID-19 vaccine. Although endomyocardial biopsy was not performed, the clinical and cardiac MRI features were consistent with myocarditis, as was the resolution of symptoms and gradual improvement in cardiac MRI findings. The case does not prove a causal association between the vaccine and the observed myocarditis-like syndrome. However, ischemic injury and other potential causes of acute myocardial injury were excluded, as were other potential infectious causes of myocarditis, and there was no evidence of systemic autoimmune disease.

The US Centers for Disease Control and Prevention have received reports of possible cases of vaccine-associated myocarditis through the VAERS reporting system, and anecdotal cases have been recently been reported in the lay media. Moreover, 2 case series in this issue of Circulation report similar presentations of myocarditis-like illness 2 to 4 days after COVID-19 vaccination.29,30 However, the link between COVID-19 vaccination and myocarditis remains circumstantial, and a mechanism has not been established. This case represents one of the first reports of possible mRNA-based COVID-19 vaccine–associated myocarditis reported in the medical literature, with in-depth clinical and translational investigation and comparison with different control groups.

Although multiple cytokines and autoantibodies with plausible links to myocarditis or cardiac pathogenesis appeared to differ in the case patient compared with controls, a specific signature was not identified. There was an increase in numbers of a specific subset of NK cells and increased expression of several autoantibodies compared with controls. T helper 17 cells–related IL-17–enriched immune signature has been implicated in the development of myocarditis and its associated transition of fibrosis to heart failure.31 It is interesting that such upregulation of IL-17 levels was not observed in our patient. The lack of evidence for upregulation of this cytokine, combined with the increased NK cell numbers observed in the case patient, could suggest a distinct vaccine-associated immunophenotype with a high likelihood for rapid recovery. However, it is not clear whether the observed differences reflect a potential (causal) pathological immune response or rather appropriate healing responses to myocardial inflammation. These differences may also be chance findings, given the exploratory nature of our investigations and large numbers of tests performed in few patients. Additional studies in larger numbers of individuals are needed to explore potential mechanisms, including prospective studies with biospecimen collection before and after vaccination.

Clinicians should be aware that myocarditis may be present in patients exhibiting cardiac signs and symptoms 2 to 4 days after COVID-19 vaccination. However, we emphasize that this report of a rare potential vaccine-related adverse event does not change the highly favorable risk/benefit of COVID-19 vaccination, including in patients with underlying heart disease or cardiomyopathy. Additional surveillance of such adverse events after COVID-19 vaccination will help to identify whether there are subgroups who are at higher risk for this vaccine-related effect, and if so, whether additional precautions are necessary.

Acknowledgments

The authors thank the Abbott Diagnostics Division in Illinois for providing SARS-CoV-2–specific antibody test kits for clinical research.

Sources of Funding

Dr Muthukumar has received grant support from Abbott and Roche Diagnostics. The autoantibody assay was funded through the Alfred W. Harris, MD, Professorship in Cardiology awarded to Dr Mammen. Dr de Lemos has received grant support from Abbott Diagnostics and Roche Diagnostics.

Disclosures

Dr de Lemos has received consulting income from Siemens Health Care Diagnostics, Ortho Clinical Diagnostics, and Quidel, Inc. The other authors report no conflicts.

Supplemental Materials

Expanded Methods

Supplementary Material

cir-144-487-s001.pdf (112.2KB, pdf)

Footnotes

*

A. Muthukumar and M. Narasimhan contributed equally.

The Data Supplement, podcast, and transcript are available with this article at https://www.ahajournals.org/doi/suppl/10.1161/CIRCULATIONAHA.121.056038.

For Sources of Funding and Disclosures, see page 497.

Contributor Information

Alagarraju Muthukumar, Email: [email protected].

Madhusudhanan Narasimhan, Email: [email protected].

Quan-Zhen Li, Email: [email protected].

Lenin Mahimainathan, Email: [email protected].

Imran Hitto, Email: [email protected].

Franklin Fuda, Email: [email protected].

Kiran Batra, Email: [email protected].

Xuan Jiang, Email: [email protected].

Chengsong Zhu, Email: [email protected].

John Schoggins, Email: [email protected].

James B. Cutrell, Email: [email protected].

Carol L. Croft, Email: [email protected].

Amit Khera, Email: [email protected].

Mark H. Drazner, Email: [email protected].

Justin L. Grodin, Email: [email protected].

Benjamin M. Greenberg, Email: [email protected].

Pradeep P.A. Mammen, Email: [email protected].

Sean J. Morrison, Email: [email protected].

References

  • 1.Luetkens JA, Faron A, Isaak A, Dabir D, Kuetting D, Feisst A, Schmeel FC, Sprinkart AM, Thomas D. Comparison of original and 2018 Lake Louise criteria for diagnosis of acute myocarditis: results of a validation cohort. Radiol Cardiothorac Imaging. 2019;1:e190010. doi: 10.1148/ryct.2019190010 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Su JR, McNeil MM, Welsh KJ, Marquez PL, Ng C, Yan M, Cano MV. Myopericarditis after vaccination, Vaccine Adverse Event Reporting System (VAERS), 1990-2018. Vaccine. 2021;39:839–845. doi: 10.1016/j.vaccine.2020.12.046 [DOI] [PubMed] [Google Scholar]
  • 3.Ader F, Surget E, Charron P, Redheuil A, Zouaghi A, Maltret A, Marijon E, Denjoy I, Hermida A, Fressart V, et al. Inherited cardiomyopathies revealed by clinically suspected myocarditis: highlights from genetic testing. Circ Genom Precis Med. 2020;13:e002744. doi: 10.1161/CIRCGEN.119.002744 [DOI] [PubMed] [Google Scholar]
  • 4.Cavalli G, Foppoli M, Cabrini L, Dinarello CA, Tresoldi M, Dagna L. Interleukin-1 receptor blockade rescues myocarditis-associated end-stage heart failure. Front Immunol. 2017;8:131. doi: 10.3389/fimmu.2017.00131 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Song T, Jones DM, Homsi Y. Therapeutic effect of anti-IL-5 on eosinophilic myocarditis with large pericardial effusion. BMJ Case Rep. 2017;2017:bcr2016218992. doi: 10.1136/bcr-2016-218992 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Diny NL, Baldeviano GC, Talor MV, Barin JG, Ong S, Bedja D, Hays AG, Gilotra NA, Coppens I, Rose NR, et al. Eosinophil-derived IL-4 drives progression of myocarditis to inflammatory dilated cardiomyopathy. J Exp Med. 2017;214:943–957. doi: 10.1084/jem.20161702 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Zhang J, Yang Z, Liang Z, Wang M, Hu C, Chang C, Shi L, Ji Q, Liu L. Anti-interleukin-16-neutralizing antibody attenuates cardiac inflammation and protects against cardiac injury in doxorubicin-treated mice. Mediators Inflamm. 2021;2021:6611085. doi: 10.1155/2021/6611085 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Nogueira LG, Santos RH, Ianni BM, Fiorelli AI, Mairena EC, Benvenuti LA, Frade A, Donadi E, Dias F, Saba B, et al. Myocardial chemokine expression and intensity of myocarditis in Chagas cardiomyopathy are controlled by polymorphisms in CXCL9 and CXCL10. PLoS Negl Trop Dis. 2012;6:e1867. doi: 10.1371/journal.pntd.0001867 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Zou Y, Takano H, Mizukami M, Akazawa H, Qin Y, Toko H, Sakamoto M, Minamino T, Nagai T, Komuro I. Leukemia inhibitory factor enhances survival of cardiomyocytes and induces regeneration of myocardium after myocardial infarction. Circulation. 2003;108:748–753. doi: 10.1161/01.CIR.0000081773.76337.44 [DOI] [PubMed] [Google Scholar]
  • 10.Yücel Ç, Sertoğlu E, Firat Oğuz E, Hayran Y, Omma A, Özgürtaş T. Serum VEGF-A and VEGFR-1 levels in patients with adult immunoglobulin A vasculitis. Int J Rheum Dis. 2021;24:789–794. doi: 10.1111/1756-185X.14115 [DOI] [PubMed] [Google Scholar]
  • 11.Hoffmann JC, Patschan D, Dihazi H, Müller C, Schwarze K, Henze E, Ritter O, Müller GA, Patschan S. Cytokine profiling in anti neutrophil cytoplasmic antibody-associated vasculitis: a cross-sectional cohort study. Rheumatol Int. 2019;39:1907–1917. doi: 10.1007/s00296-019-04364-y [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Kasama T. Elevated serum levels of macrophage migration inhibitory factor and their significant correlation with rheumatoid vasculitis disease activity. Mod Rheumatol. 2012;22:59–65. doi: 10.1007/s10165-011-0466-z [DOI] [PubMed] [Google Scholar]
  • 13.Toplak N, Kveder T, Trampus-Bakija A, Subelj V, Cucnik S, Avcin T. Autoimmune response following annual influenza vaccination in 92 apparently healthy adults. Autoimmun Rev. 2008;8:134–138. doi: 10.1016/j.autrev.2008.07.008 [DOI] [PubMed] [Google Scholar]
  • 14.Tiede A, Sachs UJ, Czwalinna A, Werwitzke S, Bikker R, Krauss JK, Donnerstag FG, Weißenborn K, Höglinger GU, Maasoumy B, et al. Prothrombotic immune thrombocytopenia after COVID-19 vaccine [published online April 28, 2021]. Blood. doi: 10.1182/blood.2021011958 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Bastard P, Rosen LB, Zhang Q, Michailidis E, Hoffmann HH, Zhang Y, Dorgham K, Philippot Q, Rosain J, Béziat V, et al. ; HGID Lab; NIAID-USUHS Immune Response to COVID Group; COVID Clinicians; COVID-STORM Clinicians; Imagine COVID Group; French COVID Cohort Study Group; Milieu Intérieur Consortium; CoV-Contact Cohort; Amsterdam UMC Covid-19 Biobank; COVID Human Genetic Effort. Autoantibodies against type I IFNs in patients with life-threatening COVID-19. Science. 2020;370:eabd4585. doi: 10.1126/science.abd4585 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Lund KP, von Stemann JH, Eriksson F, Hansen MB, Pedersen BK, Sørensen SS, Bruunsgaard H. IL-10-specific autoantibodies predict major adverse cardiovascular events in kidney transplanted patients - a retrospective cohort study. Transpl Int. 2019;32:933–948. doi: 10.1111/tri.13425 [DOI] [PubMed] [Google Scholar]
  • 17.Garcia AG, Wilson RM, Heo J, Murthy NR, Baid S, Ouchi N, Sam F. Interferon-γ ablation exacerbates myocardial hypertrophy in diastolic heart failure. Am J Physiol Heart Circ Physiol. 2012;303:H587–H596. doi: 10.1152/ajpheart.00298.2012 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Borst O, Schaub M, Walker B, Sauter M, Muenzer P, Gramlich M, Mueller K, Geisler T, Lang F, Klingel K, et al. CXCL16 is a novel diagnostic marker and predictor of mortality in inflammatory cardiomyopathy and heart failure. Int J Cardiol. 2014;176:896–903. doi: 10.1016/j.ijcard.2014.08.033 [DOI] [PubMed] [Google Scholar]
  • 19.Koitabashi N, Arai M, Niwano K, Watanabe A, Endoh M, Suguta M, Yokoyama T, Tada H, Toyama T, Adachi H, et al. Plasma connective tissue growth factor is a novel potential biomarker of cardiac dysfunction in patients with chronic heart failure. Eur J Heart Fail. 2008;10:373–379. doi: 10.1016/j.ejheart.2008.02.011 [DOI] [PubMed] [Google Scholar]
  • 20.Zhong P, Peng J, Bian Z, Huang H. The role of cold inducible RNA-binding protein in cardiac physiology and diseases. Front Pharmacol. 2021;12:610792. doi: 10.3389/fphar.2021.610792 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Mascaro-Blanco A, Alvarez K, Yu X, Lindenfeld J, Olansky L, Lyons T, Duvall D, Heuser JS, Gosmanova A, Rubenstein CJ, et al. Consequences of unlocking the cardiac myosin molecule in human myocarditis and cardiomyopathies. Autoimmunity. 2008;41:442–453. doi: 10.1080/08916930802031579 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Myers JM, Alvarez KM, Reim S, Bentley R, Garman L, Chen A, Wiley G, Bebak M, Mongomery CG, Gaffney P, et al. Molecular mimicry and signaling by human monoclonal autoantibody derived from human myocarditis and heart failure may contribute to fibrosis and remodeling in cardiomyopathy. J Immunol. 2018;200(1 suppl):166.49. https://www.jimmunol.org/content/200/1_Supplement/166.49. [Google Scholar]
  • 23.Burbelo PD, Iadarola MJ, Keller JM, Warner BM. Autoantibodies targeting intracellular and extracellular proteins in autoimmunity. Front Immunol. 2021;12:548469. doi: 10.3389/fimmu.2021.548469 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24.Suurmond J, Diamond B. Autoantibodies in systemic autoimmune diseases: specificity and pathogenicity. J Clin Invest. 2015;125:2194–2202. doi: 10.1172/JCI78084 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH. The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986;136:4480–4486. [PubMed] [Google Scholar]
  • 26.Briones MR, Morgan GA, Amoruso MC, Rahmani B, Ryan ME, Pachman LM. Decreased CD3-CD16+CD56+ natural killer cell counts in children with orbital myositis: a clue to disease activity. RMD Open. 2017;3:e000385. doi: 10.1136/rmdopen-2016-000385 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.McTiernan CF, Morel P, Cooper LT, Rajagopalan N, Thohan V, Zucker M, Boehmer J, Bozkurt B, Mather P, Thornton J, et al. ; IPAC Investigators. Circulating T-cell subsets, monocytes, and natural killer cells in peripartum cardiomyopathy: results from the Multicenter IPAC Study. J Card Fail. 2018;24:33–42. doi: 10.1016/j.cardfail.2017.10.012 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Ong S, Ligons DL, Barin JG, Wu L, Talor MV, Diny N, Fontes JA, Gebremariam E, Kass DA, Rose NR, et al. Natural killer cells limit cardiac inflammation and fibrosis by halting eosinophil infiltration. Am J Pathol. 2015;185:847–861. doi: 10.1016/j.ajpath.2014.11.023 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29.Larson KF, Ammirati E, Adler ED, Cooper LT, Hong KN, Saponara G, Couri D, Cereda A, Procopio A, Cvalotti C, et al. Myocarditis after BNT162b2 and mRNA-1273 vaccination. Circulation. 2021;144:507–509. doi: 10.1161/CIRCULATIONAHA.121.055913 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Rosner CM, Genovese L, Tehrani BN, Atkins M, Bakhshi H, Chaudhri S, Damluji AA, de Lemos JA, Desai SS, Emaminia A, et al. Myocarditis temporally associated with COVID-19 vaccination. Circulation. 2021;144:503–506. doi: 10.1161/CIRCULATIONAHA.121.055891 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 31.Myers JM, Cooper LT, Kem DC, Stavrakis S, Kosanke SD, Shevach EM, Fairweather D, Stoner JA, Cox CJ, Cunningham MW. Cardiac myosin-Th17 responses promote heart failure in human myocarditis. JCI Insight. 2016;1:85851. doi: 10.1172/jci.insight.85851 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32.Narasimhan M, Mahimainathan L, Araj E, Clark AE, Markantonis J, Green A, Xu J, SoRelle JA, Alexis C, Fankhauser K, et al. Clinical evaluation of the Abbott Alinity SARS-CoV-2 spike-specific quantitative IgG and IgM assays among infected, recovered, and vaccinated groups. J Clin Microbiol. 2021;59:e0038821. doi: 10.1128/JCM.00388-21 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33.Pichilingue-Reto P, Raj P, Li QZ, Dozmorov I, Karp DR, Wakeland EK, Nelson M, Gruchalla RS, de la Morena MT, van Oers NSC. Serum IgG profiling of toddlers reveals a subgroup with elevated seropositive antibodies to viruses correlating with increased vaccine and autoantigen responses. J Clin Immunol. 20211–17. doi: 10.1007/s10875-021-00993-w [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.van Oers NSC, Hanners NW, Sue PK, Aquino V, Li QZ, Schoggins JW, Wysocki CA. SARS-CoV-2 infection associated with hepatitis in an infant with X-linked severe combined immunodeficiency. Clin Immunol. 2021;224:108662. doi: 10.1016/j.clim.2020.108662 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 35.Zaenker P, Prentice D, Ziman M. Tropomyosin autoantibodies associated with checkpoint inhibitor myositis. Oncoimmunology. 2020;9:1804703. doi: 10.1080/2162402X.2020.1804703 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Gruber CN, Patel RS, Trachtman R, Lepow L, Amanat F, Krammer F, Wilson KM, Onel K, Geanon D, Tuballes K, et al. Mapping systemic inflammation and antibody responses in multisystem inflammatory syndrome in children (MIS-C). Cell. 2020;183:982, e14–995. doi: 10.1016/j.cell.2020.09.034 [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 37.Fiorentino DF, Presby M, Baer AN, Petri M, Rieger KE, Soloski M, Rosen A, Mammen AL, Christopher-Stine L, Casciola-Rosen L. PUF60: a prominent new target of the autoimmune response in dermatomyositis and Sjögren’s syndrome. Ann Rheum Dis. 2016;75:1145–1151. doi: 10.1136/annrheumdis-2015-207509 [DOI] [PMC free article] [PubMed] [Google Scholar]

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