Figure 4. Dystrophin expression, baseline and treated part 2, week 48, biopsy samples from the same patient.
(A) Example 1: Low magnification image of whole tissue section stained for laminin α2 (green, all fibers stained) and dystrophin (red). A total of 2,843 muscle fibers were present in the baseline whole-slide image (A.a) and 1,077 fibers in the part 2, week 48, image (A.b). Three highlighted regions from the whole tissue sections (green) are magnified below each image to illustrate the corresponding dystrophin staining in these regions (red). Image intensity was not enhanced and represents original captured images. Dystrophin protein at part 2, week 48, was 4.3% of normal, as measured by Western blot for this patient. (B) Example 2: Low magnification image of whole tissue section stained for laminin α2 (green, all fibers stained) and dystrophin (red). A total of 4,945 muscle fibers were present in the baseline whole-slide image (B.a) and 2,167 fibers in the part 2, week 48, image (B.b). Three highlighted regions from the whole tissue sections (green) are magnified below each image to highlight the corresponding dystrophin staining in these regions (red). Image intensity was not enhanced and represents original captured images. Dystrophin protein at part 2, week 48, was 1.91% of normal, as measured by Western blot for this patient.