Table 2.
Sequences of PCR primers and TaqMan probes for specific feline cytokines
| Cytokine | Primer | Sequence (5′–3′) | Length | Probe | Probe sequence (5′–3′) | Accession |
|---|---|---|---|---|---|---|
| IL-1β | fIL1b.55f | AATGACCTGTTCTTTGAGGCTGAT | 76 | fIL1b.84p | CGAAAAGATGAAGGGCAGCCTCCAA | M92060 |
| fIL1b.130r | CCAGAAAACTGTGGCTCAGGTT | |||||
| IL-6 | fIL6.132f | CCCTGCAGACAAAATGGAAGA | 110 | fIL6.181p | TGTTATAGTTGTCACACATCTCCTTTTTCAGTGCAGA | X87408 |
| fIL6.241r | GTGCCTCCTTGCTGTCCTCA | |||||
| IL-18 | fIL18.178f | GGAGATCAACCTGTGTTTGAGGAT | 105 | fIL18.208p | ATTCTGACTGTACAGATAATGCACCCCGGAC | ∗ |
| fIL18.297r | GATGGTTACTGCCAGACCTCTAGTG | |||||
| IFN-α | fIFNa.112f | CTTGACGCTCCTGGGACAAA | 101 | fIFNa.141p | TCCCTGCCATCTCCTGTCAGAAGG | E04599 |
| fIFNa.212r | ACTGGTCTCCACCAGAACACG | |||||
| TNF-α | fTNFa.239f | CTTCTCGAACTCCGAGTGACAAG | 74 | fTNFa.266p | TAGCCCATGTAGTAGCAAACCCCGAAGC | M92061 |
| fTNFa.312r | CCACTGGAGTTGCCCTTCA | |||||
| MIP-1α | fMIP1a.17f | CTGCCCTCGCCATCCTC | 99 | fMIP1a.62p | TCTTCTCTGCACCATTTGGTGCTGACAC | Z14137 |
| fMIP1a.115r | ATAGGAGAAGCAGCAGGCAGTT | |||||
| RANTES | fRANTES.157f | CTACACCAGCAGCAGTGTTCC | 67 | fRANTES.180p | TGCCCAGCAGTCGTCTTTGTCACCC | U22385 |
| fRANTES.223r | ACACACCTGGCGCTTCCTC |
Intron–exon junctions are underlined.
∗
GenBank submission pending.