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Immunoprecipitation of recombinant M and M in SARS‐CoV infected cells. (A) M‐expressing BHK‐T7 cells were metabolically labeled and lysed at the indicated time. Immunoprecipitation was performed using S30‐antibody. The samples were treated with Endo H‐ or PNGase F, and subjected to SDS–PAGE and analyzed by autoradiography as described above. (B) Pulse‐chase experiment of M in SARS‐CoV‐infected Vero cells were performed as described above.
