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. 1994;134(3):421–426. doi: 10.1007/BF01310579

Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses

X Zhang 1, W Herbst 2, K G Kousoulas 1, J Storz 1
PMCID: PMC7087011  PMID: 8129626

Summary

The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9.

Keywords: Nucleotide, Western Blot, Monoclonal Antibody, Infectious Disease, Sequence Analysis

References

  • 1.Cavanagh D, Brian DA, Enjuanes L, Holmes KV, Lai MM, Laude H, Siddell SG, Spaan W, Taguchi F, Talbot PJ. Recommendations of the Corona-virus Study Group for the nomenclature of the structural proteins, mRNAs, and genes of coronaviruses. Virology. 1990;176:306–307. doi: 10.1016/0042-6822(90)90259-T. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Cox E, Pensaert MB, Callebaut P, van Deun DK. Intestinal replication of a porcine respiratory coronavirus closely related antigenically to the enteric transmissible gastroenteritis virus. Vet Microbiol. 1990;23:237–243. doi: 10.1016/0378-1135(90)90154-N. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Doughri AM, Storz J. Light and ultrastructural pathologic changes in intestinal coronavirus infection of newborn calves. Zentralbl Vet Med. 1977;24:367–385. doi: 10.1111/j.1439-0450.1977.tb01011.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Gallagher TM, Parker SE, Buchmeier MJ. Neutralization-resistant variants of a neurotropic coronavirus are generated by deletions within the amino-terminal half of the spike glycoprotein. J Virol. 1990;64:731–741. doi: 10.1128/jvi.64.2.731-741.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Heckert RA, Saif LJ, Hoblet KH, Agnes AG. A longitudinal study of bovine coronavirus enteric and respiratory infections in dairy calves in two herds in Ohio. Vet Microbiol. 1990;22:187–201. doi: 10.1016/0378-1135(90)90106-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Herbst W, Klatt E, Schliesser T. Serologisch-diagnostische Untersuchungen zum Vorkommen von Coronavirusinfektionen bei Atemwegserkrankungen des Rindes. Berl Münch Tierärztl Wochenscht. 1989;102:129–131. [PubMed] [Google Scholar]
  • 7.Herrler G, Rott R, Klenk HD. Neuraminic acid is involved in the binding of influenza C virus to erythrocytes. Virology. 1985;141:144–147. doi: 10.1016/0042-6822(85)90190-4. [DOI] [PubMed] [Google Scholar]
  • 8.Hussain K, Storz J, Kousoulas KG. Comparison of bovine coronavirus (BCV) antigens: Monoclonal antibodies to glycoprotein gp 100 distinguish between vaccine and wild-type strains. Virology. 1991;183:442–445. doi: 10.1016/0042-6822(91)90163-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Jimenez C, Herbst W, Biermann U, Müller JM, Schliesser T. Isolierung von Coronaviren in der Zellkultur aus Nasentupferproben atemwegskranker Kälber in der Bundesrepublik Deutschland. J Vet Med B. 1989;36:635–638. [PubMed] [Google Scholar]
  • 10.Kingston RE. Guanidinium method for total RNA preparation. In: Ausubel FM, editor. Current protocols in molecular biology. New York: Greene Wiley-Interscience; 1989. pp. 421–425. [Google Scholar]
  • 11.Mebus CA, Stair EL, Rhodes MB, Twiehaus MJ. Pathology of neonatal calf diarrhea induced by a coronavirus-like agent. Vet Pathol. 1973;10:45–64. doi: 10.1177/030098587301000105. [DOI] [PubMed] [Google Scholar]
  • 12.Mebus CA, Stair EL, Rhodes MB, Twiehaus MJ. Neonatal calf diarrhea: propagation, attenuation and characteristics of coronavirus-like agents. Am J Vet Res. 1973;34:145–150. [PubMed] [Google Scholar]
  • 13.Möstl K, Bürki F. Ursächliche Beteiligung boviner Coronaviren an respiratorischen Krankheitsausbrüchen bei Kälbern und pathogenetisch-immunologische Überlegungen hierzu. Dtsch Tierärztl Wochenschr. 1988;95:19–22. [PubMed] [Google Scholar]
  • 14.Pensaert M, Callebaut P, Vergote J. Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. Vet Q. 1986;8:257–261. doi: 10.1080/01652176.1986.9694050. [DOI] [PubMed] [Google Scholar]
  • 15.Rai RB, Singh NP. Isolation of coronavirus from neonatal calves with pneumoenteritis in India. Vet Rec. 1983;113:47–48. doi: 10.1136/vr.113.2.47-a. [DOI] [PubMed] [Google Scholar]
  • 16.Rasschaert D, Duarte M, Laude H. Porcine respiratory coronavirus differs from transmissible gastroenteritis virus by a few genomic deletions. J Gen Virol. 1990;71:2599–2607. doi: 10.1099/0022-1317-71-11-2599. [DOI] [PubMed] [Google Scholar]
  • 17.Reynolds DJ, Debney TG, Hall GA, Thomas LH. Studies on the relationship between coronaviruses from the intestinal and respiratory tracts of calves. Arch Virol. 1985;85:71–83. doi: 10.1007/BF01317007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Saif LJ, Redman DR, Moorhead PD, Theil KW. Experimentally induced coronavirus infections in calves: viral replication in the respiratory and intestinal tracts. Am J Vet Res. 1986;47:1426–1432. [PubMed] [Google Scholar]
  • 19.Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA. 1977;74:5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Schultze B, Wahn K, Klenk HD, Herrler G. Isolated HE-protein from hemagglutinating encephalomyelitis virus and bovine coronavirus has receptor-destroying and receptor-binding activity. Virology. 1991;180:221–228. doi: 10.1016/0042-6822(91)90026-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Schultze B, Gross HJ, Brossmer R, Herrler G. The S protein of bovine coronavirus is a hemagglutinin recognizing 9-0- acetylated sialic acid as a receptor determinant. J Virol. 1991;65:6232–6237. doi: 10.1128/jvi.65.11.6232-6237.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Spaan W, Cavanagh D, Horzinek MC. Coronaviruses: structure and genome expression. J Gen Virol. 1988;69:2939–2952. doi: 10.1099/0022-1317-69-12-2939. [DOI] [PubMed] [Google Scholar]
  • 23.St Cyr-Coats K, Storz J. Bovine coronavirus induced cytopathic expression and plaque formation: host cell and virus strain determine trypsin dependence. J Vet Med B. 1988;35:48–56. doi: 10.1111/j.1439-0450.1988.tb00465.x. [DOI] [PubMed] [Google Scholar]
  • 24.Sturman LS, Ricard CS, Holmes KV. Proteolytic cleavage of the E2 glycoprotein of murine coronavirus: activation of cell-fusing activity of virions by trypsin and separation of two different 90K cleavage fragments. J Virol. 1985;56:904–911. doi: 10.1128/jvi.56.3.904-911.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Tompkins WAF, Watrach AM, Schmale JD, Schultz RM, Harris JA. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J Natl Cancer Inst. 1974;52:904–911. doi: 10.1093/jnci/52.4.1101. [DOI] [PubMed] [Google Scholar]
  • 26.Zhang XM, Kousoulas KG, Storz J. Comparison of the nucleotide and deduced amino acid sequences of the S genes specified by virulent and avirulent strains of bovine coronaviruses. Virology. 1991;183:397–404. doi: 10.1016/0042-6822(91)90154-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Zhang XM, Kousoulas KG, Storz J. The hemagglutinin/esterase glycoprotein of bovine coronaviruses: sequence and functional comparisons between virulent and avirulent strains. Virology. 1991;185:847–852. doi: 10.1016/0042-6822(91)90557-R. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Zhang XM, Kousoulas KG, Storz J. The hemagglutinin/esterase gene of human coronavirus strain OC43: phylogenetic relationships to bovine and murine coronaviruses and influenza C virus. Virology. 1992;186:318–323. doi: 10.1016/0042-6822(92)90089-8. [DOI] [PMC free article] [PubMed] [Google Scholar]

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