Figure 3.
Intracerebral hematoma induces brain tissue inflammation. (A) H&E staining for histological observation of perihematomal tissues (Model) and normal contralateral cerebral tissues (Normal) was performed at 12 h, 24 h and 72 h after ICH (Original magnification ×200). Hemorrhage distribution tended to disperse from 12 h to 24 h and decrease at 72 h. There is no obvious change in the normal tissue. Relative expression of IL-1β (B), IL-6 (C), IL-8 (D), IRAK1 (E) and TNF-α (F) in the perihematomal tissue and normal contralateral cerebral tissues at 12 h, 24 h and 72 h post-ICH was measured by qPCR. Relative expression levels were normalized to endogenous GAPDH, with data represented as mean ± SD, n=6. **P<0.01, ***P<0.001 versus Normal group. The expression of IL-1β, IL-6, and IRAK increased at all time-tested points and peaked at 12 h in the perihematomal tissue. IL-8 expression started to increase at 24 h and peaked at 24 h. The expression of TNF-α also increased at all time-tested points and peaked at 24 h.