Fig. 7.
Upstream mechanism that regulates specific expression of Foxg. a–c Foxg expression in embryos injected with a the lacZ control MO, b the Foxc MO, and c the Tfap2-r.b MO. Rows that express Foxg are shown by black arrowheads, and rows that lose the expression are shown by white arrowheads. d Immunostaining of dpERK (magenta) in an embryo in which ANB cells were marked with Foxc > Gfp. The brightness and contrast of fluorescence images were adjusted linearly. Note that dpERK signals are also seen in the most anterior row of the neural plate. e Depiction of dpERK-positive cells in the ANB. f–i In situ hybridization of f, g Foxg and h, i Emx in f, h control embryos and g, i embryos treated with U0126. The number of embryos examined and the proportion of embryos that each panel represents are shown within the panels. The scale bar in (a) represents 50 μm