Fig. 2.
Fluorescence staining of cells treated with apoE3 and E4 in the presence and absence of Aβ. Primary rat pyramidal hippocampal cultures (DIV-1) were treated with control medium (A, D), apoE3 (B,E), or apoE4 (C, F) (10 μg apoE/ml). On DIV-5, 10 μm Aβ(1–40) was added to D–F, and cells were stained with fluorescein diacetate/propidium iodide on DIV-10. Source of apoE as described (Fig. 1). Typical micrographs are shown with fluorescein diacetate (yellow/green)- labeled intact cells and propidium iodide (orange)- labeled condensed or fragmented nuclei of compromised cells (arrows in C, D,F). Scale bar, 10 μm.