Fig. 1.
Immunoprecipitation of GABAA receptors solubilized from rat cerebellum. Solubilized receptors (470 fmol of [3H]Ro 15-4513 binding sites) were incubated with increasing amounts of α6(1–15) or α6(429–434) antibodies in a final volume of 350 μl. Receptors present in the pellets (solid symbols) or the supernatant (open symbols) were determined by specific [3H]Ro 15-4513 binding. Identical results were obtained when the supernatant from the α6(1–15) or α6(429–434) immunoprecipitation was investigated. The values are mean ± SD of four separate experiments performed in triplicates. SD bars that were smaller than the diameter of the symbols are not shown.