Fig. 6.
IL-6 transcription in the presence of TNF-α, IL-1β, and IL-6/sIL-6R. U373-MG cells were incubated in the presence of medium, TNF-α, IL-1β, and IL-6/sIL-6R alone and in combination for 30–45 min. Labeled transcripts were prepared as described in Materials and Methods and hybridized to filters containing 5 μg of nonspecific DNA, human IL-6 cDNA, or human GAPDH cDNA. The blots were exposed to the PhosphorImager, and IL-6 values were normalized to the value of GAPDH for each sample. These data are representative of two experiments.