Fig. 1.
Immunolocalization of mGlu2/3 in the nucleus accumbens. A, At the light microscope level, immunostaining is associated with elongated processes (arrows) and dot structures dispersed between unlabeled neuronal cell bodies (N). B–D, At the electron microscope level, immunostaining is essentially associated with axon terminals containing numerous synaptic-like vesicles (ax) and glial processes (gl), whereas dendritic profiles (D) always appear unstained. Note that labeled axon terminals frequently form typical synaptic contacts with unlabeled dendrites (arrowheads in B andC), whereas labeled glial processes frequently enwrap either labeled (C) or unlabeled (D) synaptic axon terminals.Asterisks in B–D indicate the unlabeled axon terminals. Scale bars: A, 25 μm;B–D, 1 μm.