Fig. 4.
Antibody validation. a, Strong immunoreactivity to a stable HEK293–recombinant human HCN1 cell line is seen using anti-HCN1 antibody (left,HEK+HCN1). Immunoreactivity is absent in the parent (untransfected) cell line (HEK−) using the same antibody. b, Western blot analysis shows no anti-HCN1 antibody recognition of extracts from untransfected (HEK−) cells and a specific double band pattern in HEK+HCN1 cell extracts (left). Right, Single bands are identified in DRG and stable HEK-tsA201–recombinant human HCN3 (HEK+HCN3) cell line extracts using anti-HCN3 antibody, with absence of staining of HEK− cells.