Fig. 3.
Distribution and quantification of HCN mRNAs after nerve injury. a, In situ hybridization: comparisons of co-embedded contralateral (uninjured) and ipsilateral (injured) L5 DRGs hybridized with antisense probes for HCN1, HCN2, or HCN3 show a visible reduction in both HCN1 and HCN2 message. No hybridization was seen using sense probes. Scale bar, 50 μm.b, Relative quantification of HCN mRNA transcripts in sham versus SNL L5/6 DRGs (normalized copy number per 2 DRGs) by real-time fluorescent quantitative PCR. In the SNL samples, there is a significant reduction in a 3′ but not in a 5′ HCN1 amplicon compared with controls, a significant reduction in HCN2, and no change in HCN3 or HCN4 (*p < 0.02, unpaired ttest; n = 8 each group).