Figure 3.
Amyloid plaque formation in the prefrontal cortex and cerebral cortex of APPSwe/PS1 and APPSwe/PS1/CCR2−/− mice. Anti-Aβ immunoreactivity is depicted in the prefrontal cortex (left column) and cerebral cortex (right column) of APPSwe/PS1 (a, b) and APPSwe/PS1/CCR2−/− mice (c, d) at 9 months of age. A detailed analysis of plaque quantification was performed to determine plaque density (e, f), plaque size (g, h), and the area percentage occupied by plaques (i, j) of 3- to 12-month-old APPSwe/PS1 (open bar) and APPSwe/PS1/CCR2−/− mice (solid bar). Amyloid plaque formation was similar in both groups at 3, 6, and 9 months of age, although Aβ plaque load significantly increased in the prefrontal cortex of 12-month-old APPSwe/PS1/CCR2−/− mice. Results are expressed as mean ± SEM; n = 7–15; Student's t test; *p < 0.05 vs APPSwe/PS1 at the same age. Scale bar, 500 μm.