Fig. 7.
Relationship between time course of inhibition induced by puff application of dynorphin and free diffusion of dynorphin. A, Dose–response relationship of dynorphin A(1–17) (squares), dynorphin A(1–8) (up triangles), dynorphin B (down triangles), and α-neoendorphin (diamonds) on mossy fiber responses. Each point represents the mean ± SEM of four to seven experiments and is expressed as the percentage of depression of control responses. The fitting curves were drawn according to the logistic equation described in Results. Also shown is the lack of effect of a cocktail of peptidase inhibitors on the depression of mossy fiber responses induced by low concentration (10–100 nm) of dynorphin A(1–17) (open circles; n = 4). B, The time course of dynorphin action is represented by the filled circles (n = 6; same data as the puff data in Fig. 6A), and the family of curves was drawn according to a formula combining Fick’s and logistic equations (see text).