Figure 3.
Septal and hippocampal NGF levels in Ts65Dn mice. ELISA measurements of NGF (nanogram of NGF per gram of tissue, wet weight; mean ± SEM) showed significant increases in the hippocampus of both 2N and Ts65Dn mice between 6 and 12 months of age. NGF levels were significantly higher in the hippocampus of Ts65Dn (black) vs. 2N (white) mice at 6 months of age (2N = 3.4 ± 0.3; Ts65Dn = 5.5 ± 0.5, P = 0.04, n = 5) but were not significantly different at 12 months of age (2N = 5.9 ± 1.6; Ts65Dn = 8.7 ± 1.9; P = 0.15, n = 4). There was a trend toward a reduction in the level of NGF in the septal region of Ts65Dn mice at 6 months of age (2N = 1.7 ± 0.2; Ts65Dn = 1.0 ± 0.2; P = 0.18, n = 5) and at 12 months of age (2N = 3.0 ± 0.2; Ts65Dn = 1.4 ± 0.3; P = 0.25, n = 4), but these changes did not reach statistical significance.