Fig. 3.
a AT8 stained parietal section indicating the four sample areas. ai 3 × 3 large image acquisition of cortex with AT8-IR. aii Image ai with applied bespoke threshold for AT8-IR only. Mean area covered by IR was stated as a percentage of the total image area and the respective values are expressed as AT8-IR. b H&E stained section of parietal tissue with deep white matter indicated (dashed line). Microphotoimages of up to eight individual white matter vessels were captured from the white matter for SI assessment. bi A microphotoimage of a white matter arteriole. SI was calculated using VasCalc software; [66] the internal and external diameters were measured three times to yield a SI value a mean SI value was calculated per section. IR immunoreactivity, SI sclerotic index, Dext external diameter, Dint internal diameter. Scale bar 1 cm, valid for images in a, b; 100 μm, valid for ai, aii; 50 μm, valid for bi