Figure 1. The effect of zoledronic acid on the prenylation of human NSCLC cell lines with mutant and wild type KRAS.
Cell suspensions were separated into membrane and cytosol fractions by ultracentrifuge. Western blot confirmed that compared to control (C), treatment with 25 μM zoledronic acid (Z) could not modify membrane-association of mutant K-Ras in H358 and A549, while in LCLC-103H prenylation of wild type K-Ras was inhibited. H1975 cells showed approx. 50 percentage decrease, while zoledronic acid did not inhibit prenylation of wild type K-Ras in H1650 cells. Na/K-ATPase was applied as loading control.