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Colocalization of SARS coronavirus replicase products with de novo-synthesized viral RNA. SARS coronavirus-infected cells were transfected with Lipofectamine containing BrUTP at 21 h postinfection. Cells were treated with actinomycin D (5 μg/ml) to block cellular mRNA synthesis. The cells were double stained with rabbit polyclonal antisera against nsp1 (anti-R1), nsp2 (anti-R2), or nsp3 (anti-R3) and a mouse monoclonal antibromodeoxyuridine antibody to detect newly synthesized RNA.
