Figure 4.
Enrichment of Alpha and Beta Cells Based on Cell-Surface Markers
(A) t-SNE map highlighting the combined expression of CD24 and CD44.
(B) Results of FACS enrichment based on selection against CD24/CD44. Two libraries were selected for live staining (/), and four were selected against CD24 and CD44 expression (−/−). The y axis indicates the portion of the library consisting of a particular cell type. Colors indicate cell types.
(C) t-SNE map highlighting the expression of TM4SF4.
(D) Imagestream analysis of dispersed, fixed single-cells from human pancreas. The left panels show a bright field image of the cell and then immunostaining against glucagon (green) and Tm4sf4 (red). The lower panel shows insulin in green.
(E) t-SNE map highlighting libraries from a TM4SF4/CD24 sort. Cells that were not stained are in pink. Cells sorted for TM4SF4+/CD24− are in green and for TM4SF4−/CD24− are in blue.
(F) Results of FACS enrichment based on selection for TM4SF4/CD24. Four libraries were selected for live staining (/). Two libraries were TM4SF4+/CD24− (+/−), and two were TM4SF4−/CD24− (−/−). The y axis indicates the portion of the library consisting of a particular cell type. Colors indicate cell types.