Figure 3. TCDD induces Ahr-dependent CpG demethylation of the Cyp1a1 promoter.
Animals were treated with 3 μg/kg TCDD and livers were sampled at the indicated time points. (a,b) The methylation level of the −500 CpG and −420 CpG, respectively, in the Cyp1a1 promoter as measured by MSRE-qPCR. Data are expressed as mean ± SE. (n = 3). *P < 0.05, two-way ANOVA followed by Fisher's PLSD post hoc test.