Extended Data Figure 4. Evolution of virus infectivity is constrained by CRISPR diversity when CRISPR immune populations compete with sensitive CRISPR KO bacteria.
Emergence of virus mutants that overcome CRISPR-mediated immunity during the experiment shown in Extended Data Fig. 3. Each column in a table represents a time point (0, 16, 24, 40, 48, 64 and 72 h.p.i., as indicated below the table (in days post-infection)) where virus was isolated. Green indicates no escape virus. Red indicates emergence of escape virus. Panels a–e correspond to each of the experiments shown in Extended Data Fig. 3a–e. Bold numbers indicate each of the individual biological replicates, as detailed in the legend of Extended Data Fig. 3. In b–e, individual replicates are separated by bold lines. Numbers between parentheses indicate the identity of clones that are present in a population with CRISPR-mediated immunity. Asterisks indicate replicate experiments where virus went extinct during the experiment.