Extended Data Figure 7. Analysis of WT→WT and WT→RarHet bone marrow chimeras.
Two week-old CD45.2 RarHet and WT littermate controls were lethally irradiated and transplanted with WT CD45.1 bone marrow cells. Chimeric mice were analysed 8 weeks after reconstitution. a, Reconstitution of donor CD45.1 cells in WT→WT and WT→RarHet chimeras in the spleen (n=4). b, Reconstitution of donor CD45.1 CD4 and CD8 T cells in WT→WT and WT→RarHet chimeras in the spleen. WT→WT n=4; WT→RarHet n=11. c, Reconstitution of donor CD45.1 CD11c+MHCII+ and CD11b+Gr1+ myeloid cells in WT→WT and WT→RarHet chimeras in the spleen; n=3. d, Reconstitution of donor CD45.1 CD11c+MHCII+ and CD11b+Gr1+ cells in WT→WT and WT→RarHet chimeras in intrathoracic LNs; n=3. e, Dendritic cells (DCs) were purified from WT→WT and WT→RarHet chimeras. DCs were loaded with OVA peptide (10−5 μM) and co-cultured for 3 days with CFSE-labelled monoclonal OT1 CD8 T cells. OT1 CD8 T cell proliferation was analysed by CFSE dilution. Proliferation index is shown. WT n=4; RarHet n=6. f, Dendritic cells (DCs) were purified from WT→WT and WT→RarHet chimeras. DCs were loaded with OVA peptide (10−5 μM) and co-cultured for 3 days with OT1 CD8 T cells. Percentage of IFNγ producing OT1 CD8 T cells is shown; n=4. Error bars show s.e.. Two tailed t-test p values are indicated. *P<0.05; **P<0.01; ***P<0.001. n.s., not significant.