Fig. 3.
Co-localization of the spike protein with vimentin in Vero E6 cells pre-treated with SARS-CoV VLPs. Vero E6 cells pre-treated with SARS-CoV VLPs for 10 min were fixed for immunofluorescence assay with anti-spike antibodies followed by AlexaFluor 594-conjugated goat anti-rabbit IgG and anti-vimentin antibodies followed by AlexaFluor 488-conjugated goat anti-mouse IgG antibodies as shown in red and green colors, respectively. Vero E6 cells without the pretreatment with SARS-CoV VLPs and ACE2-negative HEK293T cells treated with SARS-CoV VLPs for 10 min were used as controls. Hoechst staining was performed in parallel to localize cell nuclei in the field and the distribution of vimentin in cytosol was examined using permeable Vero E6 cells