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Integration of SCoVN gene into the P.pastoris GS115 confirmed by PCR using 5’ AOX1 primer and 3’ AOX1 primer (lane 1: DNA ladder, lane 2: PCR product of pPIC3.5K, lane 3: PCR product of pPIC3.5K-ScoVN, lane 4: PCR product of P.pastoris GS115 without transformation, lane 5: PCR product of control strains, lanes 6 and 7: PCR product of positive recombinants).
