Figure 7. Circadian expression of enzymes participating in TAG homeostasis and master lipid regulators in WT and Per1/2 null mice.
A., WT and Per1/2 −/− mice, fed either ad libitum or exclusively during the night were sacrificed under constant darkness, at 4 h intervals throughout the day. Total RNA was prepared from liver and mRNA expression levels of enzymes participating in TAG metabolism were determined by quantitative real-time PCR and presented as fold change relative to the lowest value. B., Circadian mRNA expression profiles of master lipid regulators in WT and Per1/2 −/− mice fed ad libitum. Data are presented as mean +/− STDEV, with a mix of 4 animals per time point. Dark grey represent the subjective night and light gray the subjective day. C., A schematic model depicting the intricate circadian regulation of hepatic TAG levels by circadian clocks and feeding behavior. Night Fed (NF). Circadian Time (CT).