FIGURE 4.
Cytoplasmic accumulation of hnRNPA1 and reduced Tnpo1 binding after quercetin treatment. A, PC-3 cells were treated with 100 μm quercetin for 18 and 24 h. Immunofluorescence analyses showed the localization of hnRNPA1 (red), and DAPI was used to stain nuclei. Merge, merged images. Scale bar, 20 μm. B, left, PC-3 cells were treated with 100 μm quercetin for 24 and 48 h. After treatment, cell lysates were immunoprecipitated with anti-hnRNPA1 antibodies, and proteins in immunoprecipitates were resolved by SDS-PAGE on 10% gels and probed with anti-Tnpo1 and anti-hnRNPA1 antibodies. In the bottom panel, one-third of the total protein lysate was loaded. Right, quantification of three independent immunoblotting analyses. The intensity of Tnpo1 was quantified by densitometry and normalized to actin. ***, p < 0.001 with respect to control. IB, immunoblot. Error bars, S.E.