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Potent in vitro neutralization of MERS-CoV by RBD-specific MAbs. (A) Neutralization of viruses pseudotyped with the MERS-CoV S glycoprotein. Pseudotyped virus was incubated with IgG1s before infection of DPP4-expressing Huh-7 cells. Luciferase activities were measured, and percent neutralization was calculated. (B) Neutralization of live virus. MERS-CoV was incubated with IgG1s before infection of Vero cells. Cytopathic effects were measured, and percent neutralization was calculated.
