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(A) Schematic structure of MERS-CoV S subunit and RBD(360-601)-Avi tag. SP, signal peptide. RBD(360-601)-Avi tag was constructed by fusing MERS-CoV residues 360 to 601 of S1 with Avi tag. (B) Polyclonal phage ELISA showing the binding of the first to fourth rounds (Rd 1 to Rd 4) of phage to RBD. Bound phage were detected with anti-M13-HRP polyclonal Ab. (C) Binding of Fabs m331 to m342 to RBD. HRP-conjugated mouse anti-FLAG tag Ab was used to detect Fab binding.
