Figure 2. Zbtb46+ cDCs are essential for survival after C. rodentium infection.
(a, b) Zbtb46DTR → WT BM chimeras were treated with 40 ng/g DT at day -3 and day -1 and splenocytes or lamina propria cells were stained for expression of the indicated markers on day 0. (a) Two-color histograms are shown for live cells pre-gated as indicated. (b) Quantification of lamina propria cDC depletion in Zbtb46DTR → WT BM chimeras from [a]. Bars represent cDC or macrophage (MΦ) cell numbers per 1 × 106 lamina propria cells. Data are from two independent experiments (error bars, SEM; n = 4 mice, Student's t-test). (c,d) The indicated BM chimeras were treated with DT (20 ng/g) one day prior to infection and every third day (5 ng/g) for the remainder of the experiment. Mice were orally inoculated with 2 × 109 c.f.u. C. rodentium and followed for survival (c) and weight loss (d). Data are from two independent experiments (error bars, SEM; n = 8 mice per group, except WT+DT n = 5 mice, survival: log-rank Mantel-Cox test, weight loss: Student's t-test). (e,f) Survival (e) and weight loss (f) of mice infected with C. rodentium. Data are from two independent experiments (error bars, SEM; Flt3l+/+ n = 10, Flt3l−/− n = 10, Ccr2+/+ n = 5, Ccr2−/− n = 4, survival: log-rank Mantel-Cox test, weight loss: Student's t-test) * P < 0.05, ** P < 0.001, NS not significant.