Fig. 5. M. bovis BCG inhibits inflammasome agonist-dependent activation of caspase-1.
A. RAW264.7 cells were treated with inflammasome agonists nigericin (20 μM) or ATP (1μM), infected with M. bovis BCG for 4h where indicated, and caspase-1 activation assessed by immunoblotting. M. bovis BCG: zmp1+ (wild type, untreated); zmp1++Cm, wild type attenuated by overnight tretment with 30 μg/ml chloramphenicol. B. Densitometric ratio of active caspase-1 (p20) to β-actin.