Figure 1. Temporal expression and phosphorylation of translation initiation factors.
(A) Temporal mRNA expression profile of translation initiation factors in mouse liver. For each time point, data are mean ± standard error of the mean (SEM) obtained from four independent animals. (B) Temporal protein expression and phosphorylation of translation initiation factors in mouse liver during two consecutive days. Western blots were realized on total or nuclear (PER2 and BMAL1) liver extracts. PER2 and BMAL1 accumulations are shown as controls for diurnal synchronization of the animals. Naphtol blue black staining of the membranes was used as a loading control. The lines through gels indicate where the images have been cropped. The zeitgeber times (ZT), with ZT0, lights on; ZT12, lights off, at which the animals were sacrificed, are indicated on each panel.