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Absence of proliferating crypts in the small intestine derived from 17.5-dpc Tcf4LoxP/LoxP_PGKCre mouse embryos. Histological analysis of the small intestine derived from 17.5-dpc mouse embryos showed the presence of proliferating/Ki67+ cells in the control tissue derived from PGKCre_Tcf4WT/WT (A) and PGKCre_Tcf4LoxP/WT (B) mice, while the proliferating/Ki67+ cells in the Tcf4Hyg/Hyg (C) and the PGKCre_Tcf4LoxP/LoxP (D) mice were completely abolished.
