Fig. 1.
Glucocorticoid-induced increase in Fkbp5 gene expression and decrease in DNAm in the AtT-20 cell line. (A) Fkbp5 gene expression increases with dexamethasone (DEX) treatment in a dose-dependent manner in the AtT-20 cell line. (B) Increase in gene expression was accompanied by statistically significant dose-dependent loss of DNAm of four CpG dinucleotides flanking the intron 5 GRE (glucocorticoid response element). (C) No dose-dependent change in DNAm was observed in the intron 1 GRE. Data are shown as mean ± SEM. * p ≤ 0.05: compared to 0 nM DEX-treated samples.