Fig. 2.
PAI-1 induction is AhR-dependent. A, AhR-negative BP8 cells were infected with a control adenovirus (AdGFP) or a virus expressing the rat AhR (AdAhRFL) at a MOI of 90 for 24 h as described previously (Elferink et al., 2001). GFP expression was used to monitor infection efficiency. B, cultures were treated with 1 μM 1-(1-propynyl)pyrene to activate the AhR for the indicated time, and Western blotting was performed on immunoprecipitated PAI-1 from the culture media to measure the secreted PAI-1 protein and on total cell lysates to assay for P4501A1 expression. Analysis of actin was used as a loading control.