Fig. 3.
Topographic and quantitative analysis of light-induced mPer1 and mPer2 in the SCN after a light pulse (900 lux, 30 min) at CT14. (A), mPer1, mPer2 and VIP expression in adjacent sections from rostral to caudal SCN are shown in two representative animals from the LP+ 90-min and LP+ 120-min groups. VIP was used as the marker for the core SCN, and light-induced mPer2 staining was used to delineate the border of the SCN. Quantitative analysis was done in the core region (VIP-containing cells region) and shell region (no VIP-containing SCN region) independently.(B), Quantitative analysis was for the relative intensity of mPer1 (left) and mPer2 (right) in core and shell SCN with (LP+) or without (LP−) the light pulse. For mPer1, the mean effect of light pulse is significant in the core region (two-way anova, P < 0.0001), but not in the shell region (P > 0.05). For mPer2, the mean effect of light-pulse is significant in both the core and the shell region (P < 0.0001). The data are presented as Mean ± SEM. Scale bar = 100 μm.