Table 1.
Blocking of M41 S1 binding to chicken respiratory tract tissuesa
Tissue type | Tissue(s) to which M41 S1 (5 μg/ml) bound in the presence of MAAI at the following concn (μg/ml)b: |
||||
---|---|---|---|---|---|
0 | 8 | 16 | 32 | 64 | |
Trachea | Goblet cells/cilia | Cilia | Cilia | Cilia | — |
Lung | Epithelium | — | — | — | — |
Tissue slides were preincubated with various concentrations of nonbiotinylated MAAI lectin before the application of Strep-Tactin-precomplexed M41 S1. No binding was observed in the presence of 64 μg/ml MAAI.
—, no binding.