Figure 1.
The nanoaxotomy lab-on-a-chip. (a) Overview of the chip with the trap system (yellow rectangle) and three recovery chambers (blue rectangle). (b) Magnified view of recovery chambers. Valves 5, 6 and 7 (yellow rectangles) work in pairs and regulate the loading of a worm to either chamber accordingly. (c) Magnified view of the trapping system. Valves 1 to 4 (yellow rectangles) respectively control inlet regulation, fine positioning of the worm (2 & 3) and gating to the recovery chambers. (d) Conceptual 3D sectional renderings of the bilayer trap channels without and with an immobilized worm. (e) Two-photon images of cross-sectional profiles of the microchannel in the trap area for increasing air pressures from 0 to 35, 70, 105, 140 and 175 kPa. (f) Cross-sectional two-photon images of a trapped worm at 105 and 140 kPa. Scale bars are 2 mm in (a) and (b), 1 mm in (c), and 50 μm in (e) and (f).