Figure 3.
Effect of SOD1 genotype on motor neuron survival. (a) GFP-positive motor neurons derived from SOD1G93A and Hb9::GFP cell lines 61 d after differentiation. (b,c) Number of GFP-positive cells derived from Hb9::GFP (b) and SOD1G93A (c) ESC lines present 15, 30, 45 and 60 d after dissociation of embryoid bodies plated at two different concentrations (8 × 105 and 4 × 105 cells per well). (d,e) Number of GFP-positive motor neurons derived from Hb9::GFP and SOD1G93A 15 and 30 d after embryoid bodies dissociation plated at concentrations of 8 × 105 (d) and 4 × 105 (e) per well. (f,g) Same experiments analyzed as percentage of GFP-positive motor neurons derived from Hb9::GFP and SOD1G93A cell lines present at 15 d still remaining at 30 d after plating at 8 × 105 (f) and 4 × 105 (g) cells per well. Error bars represent s.d.