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Derivation of Hb9::GFP;SOD1 mouse ESC lines. (a,b) PCR for human SOD1 and the internal control Il2 (a), and for GFP in Hb9::GFP, Hb9::GFP;SOD1 and Hb9::GFP;SOD1G93A ESC lines (b). (c) Expression of human SOD1 protein in Hb9::GFP, Hb9::GFP;SOD1 and Hb9::GFP;SOD1G93A ESC lines. (d) A control Hb9::GFP transgenic embryo and an E10.5 chimera generated using the Hb9::GFP ESC line.
