Fig. 5.
Effect of TNF-α or taxol-induced NF-κB reporter activity by the presence of either an inhibiting or activating antibody to TNFR1. (A) Pairs of histogram bars are results for untreated versus treated cells with 20 µg/ml TNFR1 inhibiting antibody. TNF-α-treated cells (0.1 or 1.0 ng/ml) plus the TNFR1 antibody showed inhibition (P<0.05) of NF-κB activity while the antibody did not affect the taxol (20 µM)-induced NF-κB activity. (B) An activating TNFR1 antibody did not further increase TNF-α-induced (10 ng/ml) NF-κB activity. (P<0.05). Taxol's activation of NF-κB activity was significantly less than the antibody alone or the antibody plus taxol.