Figure 2. OCT4, SOX2, and NANOG Target Genes in Human ES Cells.
(A) Venn diagram representing the overlap of OCT4, SOX2, and NANOG promoter bound regions.
(B) Representative examples of protein-coding genes co-occupied by OCT4, SOX2, and NANOG. Plots display unprocessed ChIP-enrichment ratios for all probes within a genomic region. Genes are shown to scale relative to their chromosomal position. Exons and introns are represented by thick vertical and horizontal lines, respectively. The start and direction of transcription are denoted by arrows. Green, red, and purple lines represent NANOG, SOX2, and OCT4 bound regions, respectively.
(C) OCT4, SOX2, and NANOG bind in close proximity. The distances between the midpoint of bound regions for pairs of transcription factors was calculated for the 353 regions bound by all three transcription factors. Negative and positive values indicate whether the first factor is upstream or downstream of the second factor in relation to the gene. The frequency of different distances between the bound regions is plotted as a histogram.