Fig. 3.
Contribution of individual γ1 AMP binding sites to AMPK regulation by AMP. In both panels, values are presented as mean ± SEM, n = 3–7. (A) WT AMPK and indicated β1/γ1 mutants were phosphorylated by CaMKKβ in the presence or absence of AMP. Graph shows the absolute increase in pThr172 compared to nonphosphorylated AMPK controls. **P < 0.01 and ***P < 0.001 compared to AMP-treated WT control. Immunoblot shown is a single representative experiment, vertical line indicates separate gels. (B) AMP-mediated allosteric activation of WT AMPK and indicated β1/γ1 mutants following CaMKKβ activation. *P < 0.05, **P < 0.01, and ***P < 0.001 compared to WT. Immunoblot shows pThr172 level of each sample assayed.