Fig. 2.
Deficiency of Notch2 prolongs survival and delays development of anaplastic PDAC. (A) Kaplan–Meier survival data and PDAC development of Kras, Kras;N1ko, and Kras;N2ko mice. Kras;N2ko mice have significantly prolonged survival compared with Kras and Kras;N1ko mice (P < 0.02). n.s., not significant. (B) Tumor differentiation analysis reveals more anaplastic PDAC in Kras;N2ko mice compared with Kras mice. (C) Positive X-Gal staining shows Cre-induced recombination in cells of MCN-like cysts and anaplastic PDAC in Kras;N2ko;Rosa26R+/LSL-lacZ mice. (D) Histological and immunohistochemical analysis of Kras and Kras;N2ko tumors. Expression of E-cadherin in Kras PDAC and low to absent expression in Kras;N2ko tumors. The Notch targets HES1 and PDX1 are expressed in tumors derived from both genotypes. (Scale bars: 50 μm.)