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Priming with IFN-α licenses cells to induce IFN-β gene transcription in response to SARS-CoV. RT-PCR analysis: human 293lp cells were treated with 0, 10 or 100 U recombinant human IFN-α B/D ml−1, incubated for 24 h and then infected with SARS-CoV at an m.o.i. of 1. At 16 h post-infection, total RNA was isolated from cells and assayed for the presence of mRNA for IFN-β (panel 1), the N gene of SARS-CoV (panel 2) and the cellular γ-actin gene (panel 3).
