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NEP expression in the mouse hindlimb does not affect the level of substance P, leucine-enkephalin, and bradykinin in brain or blood. Commercial ELISA kits were used to measure substance P, bradykinin, and leucine-enkephalin in brain or plasma samples prepared as described in Materials and Methods. Plasma Aβ levels were measured as described in Methods. No statistically significant difference between any groups was noted. Leu-Enk, leucine-enkephalin; mNEP, mouse neprilysin.
