Figure 2. Complementarity between crRNA and target DNA flanking sequences is required for protection.
a, Schematic of the complementarity between the flanking sequences of crRNA (top, highlighted in purple) and target DNA (bottom). The red box indicates the nucleotides mutated in the experiments shown in b. b, c, Conjugation assays of pG0400 and its mutant variants, using as a recipient the Δcrispr strain harboring different pCRISPR plasmids. Mutations are shown in red. Conjugation efficiency was determined as transconjugant cfu/recipient cfu; the average of at least 3 independent experiments is reported.