Fig. 2.
Fig. 2A. A solution of 1 g sodium carbonate, 105 μM solubilized bilirubin, saturating levels of carbon dioxide gas and compressed air, 1 mg human hemoglobin, and 42 mM hydrogen peroxide were allowed to react with constant stirring in an open system. Aliquots of reaction were taken at 0 min (square), 5 min (triangle), 10 min (X), and 30 min (diamond). Optical density was followed in the visible region. The pH of this reaction is 6.8
Fig. 2B. The apparent kinetics of bilirubin degradation and BOX production using visible spectroscopy and extinction coefficients for bilirubin and BOXes. The rise in BOXes is apparent within 10 min and plateaus at about 10 min, reaching a steady state. Bilirubin falls at 426 nm, mirroring the production of BOXes